ABSTRACT
Leishmania (Viannia) peruviana was isolated from 1/75 Lutzomyia peruensis captured during May 2006 in an endemic cutaneous leishmaniasis region of the Peruvian Andes (Chaute, Huarochiri, Lima, Peru). Sand fly gut with promastigotes was inoculated into a hamster and the remaining body was fixed in ethanol. L. (Viannia) sp. was determined by polymerase chain reaction (PCR), and Leishmania species through molecular genotyping by PCR-restriction fragment length polymorphism analyses targeting the genes cpb and hsp70, resulting L. (V.) peruviana. The infected sand fly appeared 15 days after the rains finished, time expected and useful real time data for interventions when transmission is occurring.
Subject(s)
Animals , Cricetinae , Female , Male , DNA, Protozoan/analysis , Leishmania braziliensis/isolation & purification , Psychodidae/parasitology , Genotype , Leishmania braziliensis/genetics , Peru , Polymerase Chain Reaction , Polymorphism, Restriction Fragment LengthABSTRACT
We report and illustrate two abnormal spermatheca numbers found in Peruvian sandflies, a supernumerary spermatheca in Lutzomyia cernerai and the absence of one spermatheca in L. amazonensis.
Apresentamos e ilustramos duas espermatecas de flebótomos peruanos registradas com números anormais; uma espermateca supernumerária em Lutzomyia carrerai e a falta de uma espermateca em L. amazonensis.